Using a moving window paradigm and eye tracker, the present study primarily investigated the effect of word segmentation and font size on perceptual span in Chinese reading. The experiment employed a within-subject experimental design with three factors, that is, word segmentation (no-segmentation, correct-segmentation, wrong-segmentation), font size (large, small), and window condition (L1R2, L1R3, L1R4, Full line). The results showed that there was no significant interaction between font size and window condition, nor significant interaction among word segmentation cues, font size and window condition. These findings suggest that the influence of font size on perceptual span in Chinese reading may be very limited, and word segmentation cues do not moderate this effect.