To test the measurement invariance of the College Students’ Psychological Suzhi Questionnaire Simplified Version (CSPSz-SV) across multi-groups and across times, the CSPSz-SV was used to investigate 1783 freshmen who came from 11 universities. Four hundred and forty-seven freshmen were re-investigated over six months interval. The multigroup confirmatory factor analysis (MCFA) indicated that the three-factor structure (cognition, personality, and adaptability) of the CSPSz-SV had strict measurement invariance across genders (male and female), majors (social sciences and natural sciences), families (only child and non-only child), and regions (town and country). Furthermore, the three-factor solution of the CSPSz-SV also showed strict longitudinal invariance (e.g., equality of factor patterns, factor loadings, item intercepts, and item uniqueness for all items) across six-month period. Overall, the CSPSz-SV had strict measurement invariance across genders, majors, families, and regions, as well as strict longitudinal invariance over time.